Apoptotic activity of demethoxycurcumin in MG-63 human osteosarcoma cells
강경록, 김재성, 김태현, 서정연, 박종현, 전홍성, 유선경, 김흥중, 김춘성, 김도경,
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강경록 ( Kang Kyeong-Rok ) - Chosun University Institute of Dental Science
김재성 ( Kim Jae-Sung ) - Chosun University Institute of Dental Science
김태현 ( Kim Tae-Hyeon ) - Chosun University Institute of Dental Science
서정연 ( Seo Jeong-Yeon ) - Chosun University Institute of Dental Science
박종현 ( Park Jong-Hyun ) - Chosun University Institute of Dental Science
전홍성 ( Chun Hong-Sung ) - Chosun University Department of Integrative Biological Sciences
유선경 ( Yu Sun-Kyoung ) - Chosun University Institute of Dental Science
김흥중 ( Kim Heung-Joong ) - Chosun University Institute of Dental Science
김춘성 ( Kim Chun-Sung ) - Chosun University Institute of Dental Science
김도경 ( Kim Do-Kyung ) - Chosun University Institute of Dental Science
Abstract
Demethoxycurcumin (DMC), which is a curcuminoid found in turmeric, has anti-proliferative effects on cancer cells. However, the effect of DMC on osteosarcoma has not been established. The aim of this study was to examine the effects of DMC on cell growth and apoptosis induction in MG-63 human osteosarcoma cells. This study was investigated using 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromid assay, Live/Dead cell assay, 4’, 6-diamidino-2-phenylindole staining, and immunoblotting in MG-63 cells. DMC induced MG-63 cell death in a dosedependent manner, with an estimated IC50 value of 54.4 μM. DMC treatment resulted in nuclear condensation in MG-63 cells. DMC-induced apoptosis in MG-63 cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting results showed that Bcl-2 and Bcl-xL were downregulated, while Bax and Bad were upregulated by DMC in MG-63 cells. These results indicated that DMC inhibits cell proliferation and induces apoptotic cell death in MG-63 human osteosarcoma cells via the death receptormediated extrinsic apoptotic pathway and mitochondria-mediated intrinsic apoptotic pathway.
키워드
Demethoxycurcumin; Cell death; Apoptosis; Anticancer effect
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